《植物生理学报》 2010, 46(7): 719-723

水溶性纳米量子点在玉米幼根中运输的可视化

徐建华1, 高愈希1, 赵甲庭1, 李雅2, 潘琛2, 刘志宇3, 董宇辉1,*

1 中国科学院高能物理研究所多学科中心, 北京 100049; 2 北京林业大学生物科学与技术学院, 北京 100083; 3 中国科学院生 物物理研究所计算与系统生物学研究中心, 北京 100101

通信作者：董宇辉；E-mail: dongyh@ihep.ac.cn；Tel: 010-88233090

摘　要：

在纳米生物学效应研究中, 纳米颗粒能否自由进入植物体内是一个亟待考证的关键问题。本文尝试采用一定浓度的 水溶性纳米量子点溶液培养玉米幼根, 然后徒手切片, 用荧光显微镜直接观察发光量子点在根内的运输和分布。结果表明, 量子点能够自由进入玉米根且在多种类型的细胞内都有分布; 尤其是在导管内也观察到量子点, 说明这种纳米材料能够穿 透极厚的细胞壁输送到胞内。本文也讨论了该实验设计的优缺点。

关键词：量子点; 可视化; 玉米根; 运输

收稿：2010-03-30   修定：2010-06-11

Visualization for Water-Soluble Nano Quantum Dots Transporting in Maize (Zea mays L.) Seedling Roots

XU Jian-Hua¹, GAO Yu-Xi¹, ZHAO Jia-Ting¹, LI Ya², PAN Chen², LIU Zhi-Yu³, DONG Yu-Hui^1,*

¹Center for Multi-Disciplinary Research,Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;²College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China; ³Center for Computational and Systems Biology,Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Corresponding author: DONG Yu-Hui; E-mail: dongyh@ihep.ac.cn; Tel: 010-88233090

Abstract:

In the study of nano-biological effect, it is urgent to find evidence for that if nanoparticles can transport into a plant freely. In this paper, maize seedlings are cultured in Hogland’s neutrient liquid containing water-soluble quantum dot. Five days later, the seedling roots are sliced by hands and watched by fluorescence microscopy in real time. Results show that the quantum dots can transport freely into the maize roots and distribute in various type cells, especially in the vessel cell, indicating the nanoparticles can penetrate through the extreme thick cell wall and transport into the cells. We also discuss the advantages and disadvantages of this experimental design.

Key words: quantum dot; visualization; maize root; transport